Chromatography clearly explained | Bioanalytics

Understanding Chromatography: Methods and Applications Simplified In this episode, we delve into the fundamentals of chromatography, a technique used to separate different proteins from a mixture. The video covers various methods such as size exclusion chromatography (SEC), ion exchange chromatography (IEC), affinity chromatography, and hydrophobic chromatography. Using a simplified approach, it explains how these methods work, focusing on filtering by size, charge, type of binding, and hydrophobicity. The video also highlights the possibility of combining different chromatography techniques to suit specific experimental needs. 00:00 Introduction to Chromatography 01:17 Understanding Size Filtration (SEC) 03:12 Ion Exchange Chromatography (IEC) 04:41 Affinity Chromatography Explained (Binding) 06:00 Hydrophobic Interaction Chromatography (HIC) 07:17 Combining Chromatography Methods